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miniDAWN® Static Light Scattering Instrument

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Code: #30006

Supplier: LMS Technologies Pte Ltd

miniDAWN® Static Light Scattering Instrument

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  • The miniDAWN® determines absolute molar mass and size to characterize proteins, peptides, small polymers and nanoparticles in solution. Coupled to size exclusion chromatography (SEC or GPC), it eliminates the uncertainty and hassle of column calibration, for true confidence in macromolecular characterization.
     
    The miniDAWN® not only offers superb sensitivity—it further enhances productivity thanks to some unique capabilities: field serviceability, upgradeability to UHPLC and One-Click Mw™.
     
    Included with each miniDAWN® is a copy of ASTRA, the most versatile software package available for analysis of online multi-angle and dynamic light scattering. ASTRA and the miniDAWN® incorporate advanced ease-of-use features that help guarantee excellent measurements with a single click.

  • Molar Mass Range

    200 Da to 10 MDa1

    Molecular Size Range (rg)

    10 nm to 50 nm1, up to 150 nm with shape-specific models

    Molecular Size Range (Rh, with WyattQELS DLS module or DynaProNanoStar)

    Depends on the scattering angle:
    In flow mode, 0.5 nm – 50 nm1,2
    batch mode, 0.5 nm – 1µm1

    Sensitivity

    25 ng of 100 kDa polystyrene in THF3 500 ng of BSA in PBS4

    Fluidics

    Mobile Phase Compatibility

    All-solvent compatible (aqueous and organic). Wetted materials are 316 stainless steel, fused silica, and Kalrez.

    Optics

    Detectors

    MALS Detectors

    High-gain, high dynamic range, static light scattering detectors at 3 angles.

    Auxiliary Detectors

    Laser monitor for stabilization feedback; forward transmission monitor to correct signals for absorbing samples and to assess data quality.

    A/D Resolution

    Detector dynamic range > 16,000,000:1

    DLS Detector (optional)

    WyattQELS dynamic light scattering module installs directly inside the miniDAWN® chassis.

    Alternatively, the optical fiber pickup of the DynaProNanoStar cuvette-based DLS instrument may be installed in the miniDAWN®.

    Laser Properties

    Laser Wavelength

    658 nm (nominal). Each instrument is individually characterized.

    Laser Power Control

    Programmable 10% - 100%

    Flow Cells

    Fused Silica, optimal for solvent refractive index < 1.50

    Electronics

    Analog Inputs

    2 differential analog inputs with 24 bit resolution; Input range -10 V to +10 V

    Analog Outputs

    1 analog output from user selectable measurements channels -10 V to +10 V

    Other Inputs/Outputs

    Alarm In, Alarm retransmit, Auto-Inject in, Autoinject contact closure, Recycle In, Recycle valve power

    Computer Interface

    Ethernet

    Processed Data Transmission Rate

    36.6 Hz to 0.2 Hz

    Front Panel Display

    162.5 mm, 16-bit, high resolution touch screen
     
    1-Typical. Actual range depends on dn/dc, sample concentration, sample conformation and chromatography conditions.
    2-Upper limit assumes a flow rate of 0.5 mL/min.
    3-Under HPLC-SEC conditions with 7.8x300 mm GPC columns.
    4-Under HPLC-SEC conditions with Wyatt 7.8x300 mm SEC protein columns.
     

 

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