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iXon EMCCD Cameras

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Code: #20179

Supplier: Andor Technology plc.

SRRF-Stream for Super-Resolution Radial Fluctuations

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rightMORE INFORMATION

  • SRRF is a NEW real time super-resolution microscopy functionality that operates on Andor’s iXon Life and iXon Ultra EMCCD cameras.

    Aligned with Andor’s vision that super-resolution should be easily accessible to all, ‘SRRF-Stream enabled’ cameras unlock the means to perform real time super resolution microscopy on conventional modern fluorescence microscopes. Resolution improvement from 2- to 6-fold (50-150nm final resolution) can be expected for most datasets.

     

    NEW SRRF-Stream+ Unlock Super-Resolution from your Microscope

    SRRF-Stream+ is our enhanced real time super-resolution microscopy functionality that operates on Andor’s iXon Life and iXon Ultra EMCCD cameras.

    Aligned with Andor’s vision that super-resolution should be easily accessible to all, ‘SRRF-Stream+ enabled’ cameras unlock the means to perform real time super resolution microscopy on conventional modern fluorescence microscopes. Resolution improvement from 2- to 6-fold (50-150nm final resolution) can be expected dependant on the original dataset.

    iXon Ultra 

    Driving the absolute best from EMCCD technology 

    For when only the best will do. The market leading EMCCD camera, Andor’s iXon Ultra has been long recognised as the highest performance, most versatile solution for the most demanding of light starved applications. Available for both physical and life science applications, iXon Ultra delivers unbeatable flexibility and functionality, offering market leading TE cooling, ‘2-in-1’ EMCCD and CCD readout modes and a host of other high-end functionality.

    New iXon Life

    High performance at a low price! 

    Andor’s latest iXon Life EMCCD platform is available exclusively for fluorescence microscopy applications and is engineered to deliver single photon sensitivity with absolutely unparalleled price/performance. With unmatched sensitivity, iXon Life is perfect for single molecule detection and live cell microscopy with minimized phototoxicity or photobleaching, but at a price not normally associated with high-performance EMCCD cameras.

  •  iXon Ultra iXon Life 
    Conventional CCD Amplifier  Yes -
    Integrated Shutter Yes  -
    Minimum TE Cooling Temperature -100 °C -80 °C
    Direct Data Access (CameraLink)  Yes - 
    EMCCD Mode Readout speeds (MHz) 888 model - 30, 20, 10 & 1897 model - 17, 10, 5 & 1 888 model - 30 & 10897 model - 17 & 10
    Single Photon Counting Modes Yes -
    SRRF-Stream+ CompatibleYesYes

    iXon model options 

    Each of the model types listed are available in both 'Ultra' and 'Life' platforms

    Models iXon 888 iXon 897
    Core attributes Field of view, sensitivity and speed Sensitivity and speed
    Sensor format 1024 x 1024 512 x 512
    Sensor diagonal 18.8 mm 11.6 mm
    QE Options  BV (Life) or BV, EX2, UVB (Ultra) BV (Life) or BV, EX2, UVB (Ultra)
    Pixel Size 13 µm  16 µm
    Frame Rate 26 fps (670 fps with 128 x 128 Crop Mode)  56 fps (595 fps with 128 x 128 Crop Mode)
    Read Noise < 1 e- with EM Gain  < 1 e- with EM Gain
    Pixel well depth 80,000 e- 180,000 e-
    Interface USB 3.0 USB 2.0
     

  •  

    The Electron Multiplying CCD (EMCCD) operates by amplification (EM Gain) of weak signal events to a signal level that is well clear of the read noise floor of the camera at any readout speed, rendering them single photon sensitive.

    While recent innovations in high QE, low noise sCMOS technology are certainly welcomed for broad scientific usage, through exploiting “EM gain”, EMCCD cameras offer a raw sensitivity beyond that of the latest back-illuminated sCMOS cameras. EMCCD cameras should be used for the most-light starved conditions, and unlock clear application benefits.

     
     

    Andor’s market leading iXon design ensures the absolute highest sensitivity from a quantitative scientific digital camera, particularly under fast frame rate conditions.

    Andor’s proven UltraVac™ vacuum technology, carrying a 7 year warranty, is critical to ensure both deep cooling and complete protection of the sensor. The iXon series is designed to be the most flexible yet easy to use EMCCD camera on the market, optimizable for a wide variety of application requirements in a single click via the OptAcquire™ feature. Furthermore, signal can be quantitatively calibrated in units of electrons or photons, either real time or post-processing.

     
     

    Single Molecule Detection – single molecule experiments present us with the considerable challenge of harnessing the limited photon budget from dynamic, individual fluorescent molecules, while also avoiding photobleaching. Andor iXon EMCCDs have continued to radically out-perform sCMOS detectors in this most demanding of light starved application areas.

    Super-Resolution – SRRF-Stream+ is the latest version of our real time super-resolution microscopy functionality that operates on compatible Andor cameras. ‘SRRF-Stream+ unlocks the means to perform real time super resolution microscopy on conventional modern fluorescence microscopes. Resolution improvement from 2- to 6-fold (50-150nm final resolution) can be expected dependent on image datasets. The latest version has been revised to provide even better image quality, while carrying all the original benefits of SRRF-Stream.

    Reduced phototoxicity – iXon facilitates use of lowest possible excitation power while maintaining superb signal to noise ratio, minimizing phototoxic effects. No other camera is this good at preserving your cell’s physiological behaviour over extended periods of measurement.

    Lowest fluorophore concentrations – There is an ongoing drive in fluorescence microscopy to push to lower and lower fluorophore concentrations in order not to perturb the physiology of the living cells being studied. iXon’s superior sensitivity facilitates use of unprecedentedly low label concentrations, thus minimizing the ‘observer effect’.

    Single Photon Counting – iXon Ultra presents the absolute lowest noise levels possible, providing the level of discrimination needed to confidently detect and register single photon events, ideal for demanding quantum physics applications such as entangled photon studies.

 

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