Hydrogen/deuterium exchange experiments on
proteins serve to gain information on the structure of the compounds
under investigation. The difficulty of this technique lies in the
precise adherence to incubation times and temperatures necessary for
meaningful results.
With the H/D-X PAL all these steps are
automated and therefore reproducible. Especially for large numbers of
samples and long incubation times, the nesting of steps such as addition
of labeling reagents, incubation, quenching and injection into the
HPLC-MS analyzer system, which is performed automatically by the
software, is very time-saving. All time intervals can be clocked to an
accuracy of 1 second.
The temperatures for the individual
processes, the sample storage and for the HPLC valves and separation
column can be independently controlled in the range of 1 - 40 °C.
The system can be adapted to any HPLC-MS system.