The Sepax C-Pro Cell Processing System, which is comprised of the
instrument, Sepax C-Pro protocol software, and kits, allows you to
combine multiple processing steps in a versatile manner. These steps
include but are not limited to enrichment, magnetic bead incubation,
spinoculation, concentration, washing, dilution, and splitting.
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Support your requirements and system implementation. Automated and functionally closed system to support GMP compliance.
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Simplify your operations. Compact design and user-friendly interface minimize your space and resource requirements.
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Maintain flexibility. Sepax C-Pro is a multipurpose solution allowing you to perform diverse and dedicated applications.
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Simplify record-keeping. Sepax C-Pro can transmit instrument data and
alarms for remote monitoring or for incorporation into a Chronicle eSOP.
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Keep operations running smoothly. Integrate with Chronicle manufacturing
automation software to monitor all connected instruments (Sepax C Pro
and others) and manage service records.
Cell therapy applications. The Sepax C-Pro Cell Processing Instrument
has been used to prepare CAR T cells, natural killer cells (NK cells),
and other cell types. Sepax C-Pro can be part of a robust CAR T cell
manufacturing workflow that is adaptable for cGMP compliance in
production of clinically relevant doses. In a natural killer cell
manufacturing workflow the Sepax C-Pro system was used with BeadWash
C-Pro protocol software to functionally close and automate incubation
with magnetic beads for CD3 depletion and CD56 selection. These steps
were included in a workflow with xeno-free expansion that generated 1 ×
109 primary human NK cells and can be adapted for GMP compliance.
Instrument flexibility. In combination with the appropriate protocol
software and single-use kit, the instrument can perform the following
operations:
- Concentrate cells from fresh apheresis product via plasma depletion
- Concentrate cells and remove platelets from fresh apheresis units
- Incubate magnetic beads onto the cell fractions obtained from
apheresis units – sequence concentration, platelet depletion, bead
incubation, and washing
- Enrich mononuclear cells fraction via a density gradient medium
- Concentrate, wash, and transduce isolated cells by spinoculation
- Dilute, concentrate, and wash fresh or frozen cellular product
- Dilute and split cellular products into multiple bags for accurate dosing