The Sepax C-Pro Cell Processing System, which is comprised of the
instrument, Sepax C-Pro protocol software, and kits, allows you to
combine multiple processing steps in a versatile manner. These steps
include but are not limited to enrichment, magnetic bead incubation,
spinoculation, concentration, washing, dilution, and splitting.
Support your requirements and system implementation. Automated and functionally closed system to support GMP compliance.
Simplify your operations. Compact design and user-friendly interface minimize your space and resource requirements.
Maintain flexibility. Sepax C-Pro is a multipurpose solution allowing you to perform diverse and dedicated applications.
Simplify record-keeping. Sepax C-Pro can transmit instrument data and
alarms for remote monitoring or for incorporation into a Chronicle eSOP.
Keep operations running smoothly. Integrate with Chronicle manufacturing
automation software to monitor all connected instruments (Sepax C Pro
and others) and manage service records.
Cell therapy applications. The Sepax C-Pro Cell Processing Instrument
has been used to prepare CAR T cells, natural killer cells (NK cells),
and other cell types. Sepax C-Pro can be part of a robust CAR T cell
manufacturing workflow that is adaptable for cGMP compliance in
production of clinically relevant doses. In a natural killer cell
manufacturing workflow the Sepax C-Pro system was used with BeadWash
C-Pro protocol software to functionally close and automate incubation
with magnetic beads for CD3 depletion and CD56 selection. These steps
were included in a workflow with xeno-free expansion that generated 1 ×
109 primary human NK cells and can be adapted for GMP compliance.
Instrument flexibility. In combination with the appropriate protocol
software and single-use kit, the instrument can perform the following
- Concentrate cells from fresh apheresis product via plasma depletion
- Concentrate cells and remove platelets from fresh apheresis units
- Incubate magnetic beads onto the cell fractions obtained from
apheresis units – sequence concentration, platelet depletion, bead
incubation, and washing
- Enrich mononuclear cells fraction via a density gradient medium
- Concentrate, wash, and transduce isolated cells by spinoculation
- Dilute, concentrate, and wash fresh or frozen cellular product
- Dilute and split cellular products into multiple bags for accurate dosing