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NU-5841 Direct Heat (200L) Hypoxic CO2 Incubator with Humidity and Oxygen Control

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Code: #20483

Supplier: Avidity Science Ltd.

The NU-5841 provides an easy method to monitor and control humidity and oxygen levels in the chamber for hypoxic stress studies, stem cell culture, carcinogenic tumours, toxicity, and more.

A maintenance-free Zirconia O2 ceramic sensor provides improved accuracy and lower range hypoxic control by displacement with nitrogen.

rightMORE INFORMATION

  • Suppressed oxygen environment provides control in stem cell differentiation to maintain full pluripotency of cell lines.
    • O2 Range: 0.5 to 21%
    • O2 Accuracy: ± 0.25%

    Relative Humidity (RH) Control

    An RH sensor continuously samples chamber humidity levels to monitor and control the humidity set point. If the system senses any variation in humidity, vapour is injected into the chamber by a water reservoir located underneath the chamber.

    Dual Sterilisation Cycles

    Easily accessible through the NuTouch Intelligent Interface, In-VitroCell incubators offer 145°C high heat sterilisation and a 95°C humidified decontamination cycles as a preventative measure and to eradicate contamination in the growth chamber. On-screen instructions direct users through the process. A timer function is available to delay the sterilisation process for an overnight procedure.

    NuTouch Intelligent Interface

    NuTouch is a user-friendly 5” x 7” (127 x 178 mm) colour touch screen designed for high user adoption. The electronic control system is a microcomputer designed to service the precise control requirement of the chamber environment for culture growth. The microcomputer is supported with Read-Only Memory (ROM) containing executable software, Random Access Memory (RAM) for temporary storage, and Electronically Erasable Programmable Read Only Memory (EEPROM) for control set points and parameters. The EEPROM provides for indefinite storage of these values during periods of power off or power interruption (power fault tolerant).

    NuTouch features are:

    • Temperature and Gas Monitor and Control
    • Status Indicators – Screen Identity; Run or Standby mode; Door Adjar; Air and CO2 Inject; Air and CO2 Chamber Sampling
    • Date and Time Functions in International Format
    • Password Protection
    • On-screen Icon Descriptions – Pressing on any icon will provide a description of what the item is
    • On-screen Directions – Directions are available to perform calibrations
    • Step-by-Step directions (i.e. sterilisation cycle procedure)
    • Alarm Status Menu - A red “Alarm Status” text button will appear on the Main Screen to indicate a fault within the Incubator system
    • Maintenance Required Reminders – On-screen Icon "MAINTENANCE REQUIRED" appears when a programmable reminder (0 to 48 months) hits set point to notify filter check
    • Historical Performance Monitor – Temperature and CO2 gas history is available to view on screen in graphical form or can be downloaded via USB for alarms, control systems, events, and performance information
    • Service Settings and Complete Diagnostic Controls
    • System Information
    • Display Settings 


    Constant Contamination Control (C3)

    The NU-5841 offers a unique blend of product features to promote cell growth while minimising potential contamination, creating the most intrinsic and reliable growth chamber.

    Closed Loop HEPA Filtration

    A maintenance-free air pump continuously draws environmental samples from two locations inside the chamber and passes the sample through a 99.99% HEPA filter before entering the sensor bay to measure gas and oxygen levels. The sensor bay measures gas and humidity levels from the sample to monitor before injecting back into the growth chamber. The NuTouch Electronic Control system will then determine the appropriate course of action to maintain chamber set-point conditions by placing the system on standby or injecting 99.99% HEPA filtered CO2 gas, air, nitrogen, or vapour into the growth environment.

    ISO Class 5 Cleanroom Conditions

    Based on clean room technology, the NU-5841 maintains the growth chamber at a positive pressure, similar to an ISO Class 5 Cleanroom, to slow airflow to minimise desiccation. Anytime the chamber door is open to the laboratory environment, clean HEPA filtered will be forced out instead of contaminated lab air being drawn in.

    Single Beam, Dual Wave Infrared (IR) Sensor

    A microprocessor-based, non-dispersive, single source dual wave infrared (IR) sensor monitors and controls CO2 levels inside the growth chamber. Wavelengths are only absorbed by CO2 making measurement insensitive to other components, such as water vapour. The stable IR sensor has a controlled range of 0.1 to 20% accurate within 0.1%.

    Temperature Uniformity

    The chamber walls are surrounded by five heating elements to control temperature. A door heater is separately controlled to minimise condensation on the inner chamber door. The heating elements are wrapped in a high density R5 insulation to maintain temperature integrity. Dual temperature sensor probes monitor and control temperature set points to ensure consistency and uniformity throughout the chamber.

    Incubator Humidity

    A relative humidity level of up to 90% is achieved in the Incubator by the use of a stainless steel pan placed on the bottom of the chamber. An air pump injects fresh air into the chamber at a preset duty cycle to reduce condensation forming on the chamber walls and front glass door.

    Advanced Construction

    The inner chamber is constructed of high grade polished stainless steel using crevice-free construction that provides an easily cleanable inert surface that does not promote biological growth. Seamless coved interior corners are easily cleaned promoting a healthy growth chamber. A single piece gasket creates a liquid tight seal from incubator chamber to inner door to eliminate condensation build up and potential contamination. The inner chamber shelving and gasket may be removed and routinely autoclaved if desired.

    Minimal Vibration Promotes Cell Growth

    By minimising moving parts, the NU-5841 utilises an accurate air pump to inject air and gas to eliminate potential vibration.

    A Smaller Footprint with More Useable Space

    A large 7 cubic foot (200 Litre) growth chamber comes standard with four incubator shelves with a maximum capacity of 23 shelves. By attaching heating elements directly to the chamber and insulating with High Density R5 insulation, the NU-5841 was engineered as a compact design while maximising chamber capacity.

    Optional Features

    Add CuVerro® Antimicrobial Copper Surfaces to the incubator growth chamber and shelving to kill bacteria* and minimise potential incubator contamination. CuVerro® is laboratory tested and EPA registered. Laboratory testing shows that CuVerro Antimicrobial Copper Surfaces kill more than 99.99% of bacteria within 2 hours of exposure, and continues to kill 99% of bacteria even after repeated contamination when regularly cleaned including; 

    • MRSA
    • Staphylococcus Aureus
    • Enterobacter Aerogenes
    • Pseudomonas Aeruginosa
    • E coli

  •  

    Shelf Capacity

    Dimensions (W x D) in. [mm] 19 ¾ x 18 ¾ [502 x 476]
    Supplied 4 Shelves
    Max. Capacity 23 Shelves
    Max. Weight Capacity 20 lbs. (9 kg) per Shelf
      50 lbs. (23 kg) per Incubator

     

    Utility Connections

    Gas Connections 0.25" (6.3 mm) Tubing
    Gas Input Pressure 20 PSIG (1.4 BAR) Input Pressure Maximum. Two Stage Gas Regulator Required

     

    Electrical Requirements

    Startup Power 625 watts
    Running Power 250 watts
    Sterile/Decon Cycle 995 watts
    Heat Rejected 14 BTU / minute

     

    Temperature Control System

    Sensor Type Precision Integrated Circuit
    Range 5°C to 55°C (5°C aboveambient to 30°C max. ambient)
    Accuracy ±0.1°C
    Uniformity ± 0.3°C at 37°C
    Recovery 0.12°C/minute Average
    Display Resolution 0.1°C
    Minimum Qualifications for Sterilization
    145 DEG Cycle 135°C
    95 DEG Cycle 85°C

     

    CO2 Control System

    Sensor Type Infrared single source dual wave length
    Range 0.1 to 20%. (0.0 set point idles system)
    Accuracy ± 0.1%
    Recovery Up to 5% ±0.2% / -0.5 in 5 minutes average
    Display Resolution ± 0.1%
    CO2 Control Logic Fixed/Algorithm/Manual, Environmental Adaptable

     

    Relative Humidity (RH) Control System

    Default Set-point 90%
    Range 5% above ambient to 90%
    Accuracy ± 3%
    Recovery 90% ±3% / 25 minutes

     

    Zirconia O2 Sensor

    Default Set-point 21%
    Range 0.5 to 21%
    Accuracy ± 0.25%
    Recovery 5% ±2% / 15 minutes
    Display Resolution 0.1%

    Model

    NU-5841

    Chamber Volume 7.06 ft.3 / 200 L
    Exterior Dimensions (W x D x H) 
    in. [mm]
    26.75 x 27.0 x 41.5 [679.5 x 691 x 1054]
    Chamber Dimensions (W x D x H) 
    in. [mm]
    21.15 x 21 x 26.5 [546.1 x 533.4 x 673.1]
    Weight 235 lbs. / 106 kg.

    • 200 litre chamber
    • Constant Contamination Control
    • Dual Wave Infrared (IR) Sensor
    • Dual Sterilisation Cycles (145°C Dry / 95°C Humidified)
    • Relative Humidity (RH) Sensor Control
    • Zirconia Oxygen (O2) Sensor Control 0.5 - 21%
    • NuTouch Intelligent Interface
    • Closed Loop HEPA Filtration
    • ISO Class 5 Cleanroom Conditions
    • Minimal Vibration Promotes Cell Growth

 
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