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IN Cell Analyzer 6500 HS high content analysis (HCA) System

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Code: #21899

Supplier: Cytiva

IN Cell Analyzer 6500HS is a laser-based high-content imaging system featuring IRIS confocal technology to optimize cellular imaging for all sample types and experimental goals.

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  • IN Cell Analyzer 6500HS is a laser-based high-content imaging system featuring IRIS confocal technology to optimize cellular imaging for all sample types and experimental goals.

    • EDGE confocal: Increased contrast in thick samples allows for better visualization and segmentation of biological structures.
    • Smart scanning: Spend less time imaging and reduce data load by only imaging objects of interest.
    • Biological relevance: Optimized hardware and features for 3D and live cell imaging.
    • Speed and sensitivity: Higher throughput and faster frame rates for single and multichannel imaging.

    A full confocal HCA solution

    Designed for high-throughput, speed, and sensitivity, IN Cell Analyzer 6500HS is a third-generation laser-based confocal imaging system from Cytiva. Building on the capabilities of earlier IN Cell Analyzer systems, IN Cell Analyzer 6500HS performs a wide range of high-content assays from basic multicolor fixed-cell assays to the most complex 3D- and live-cell assays. Multiple hardware and software improvements increase imaging speed and enabling acquisition of the highest quality images—even for demanding 3D samples such as spheroids. Combined with new IN Carta software, which simplifies image analysis workflows, IN Cell Analyzer 6500HS enables users of all levels to perform powerful analysis as part of a complete HCA solution from assay to answer.

  • ParameterIN Cell Analyzer 6500HS
    AutomationCompatible with most commercially available laboratory automation systems 
    CamerasCMOS 
    Height66cm / 26in 
    Width112.8cm / 44.4in mm
    Depth63.5cm / 25.0in 
    Weight107.5kg / 237lbs kg
    ObjectivesRoom for up to four objectives
    Supplied with 10×/0.45
    Objectives available from 2-100x (optional)
    Both air and water objectives available 
    Light SourceFour laser lines (405, 488, 561, 642)
    White LED for transmitted light 
    Imaging Modes

    Widefield (open aperture)
    Proprietary line scan confocal
    EDGE confocal
    Transmitted light
    DIC (optional)
    Phase contrast (optional)

     
    Pack size1 

  • Water Immersion objectives now included

    As the transition to more complex imaging applications speeds up, so does the need for improved light efficiency, resolution and flexibility of instrumentation. Imaging thick 3D cultures imbedded in aqueous media requires non-air objectives with high numerical aperture and minimal spherical aberration. Water immersion objectives are ideal to achieve maximal image quality for such samples, while reducing the scan times due to better resolving power.

    Image sample: The IN Cell Analyzer 6500HS high content analysis (HCA) System beautifully captures the 3D structure and cellular organization of an InSphero 3D InSight™ human colorectal carcinoma microtissue model produced from a HCT116 cancer cell line and fibroblast co-culture. Water immersion objectives combined with the proprietary EDGE Confocal technology provide clear volumetric data deep into the tissue.

    Biological relevance

    IN Cell Analyzer 6500HS is designed to eliminate systematic variability and give you confidence in your results. With reliable autofocus under all conditions, vibration isolation, and environmental control designed for uniformity, you can be assured that your data is a true representation of the biological phenomena in question.

    EDGE confocal

    Contrast is the most important thing to consider when automated image analysis is generating your data. The higher the contrast, the more reliable the segmentation and quantitation. The IRIS confocal design uses an adjustable virtual aperture to optimize image quality for each individual channel. Building on the benefits of IRIS, EDGE confocal technology dramatically increases contrast in thick samples while preserving the quantitative information you need for reliable analysis.

    Smart scanning

    The difference between a standard microscope and an automated HCA system built for screening is primarily a person's intervention and their time. A common approach to solving this problem is to image everything. Smart scanning features on IN Cell Analyzer 6500HS can automate the human element and reduce the total time and data load by finding structures of interest and inspecting them in more detail.

    Speed and sensitivity

    Technology is constantly evolving and IN Cell Analyzer platforms are keeping pace. With the latest sCMOS camera technology, IN Cell Analyzer 6500HS provides lower noise, shorter exposure times, and faster frame rates. In addition, hardware optimizations tailored to multichannel experiments increase both speed and throughput for 2D and 3D experiments.

 

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