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IN Cell Analyzer 2500 HS high content analysis (HCA) imaging system

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Code: #21898

Supplier: Cytiva

IN Cell Analyzer 2500HS is a widefield high-content imaging system designed for speed and throughput. Equipped with deconvolution, IN Cell Analyzer 2500HS is optimized for image quality and long-term live-cell imaging.


  • IN Cell Analyzer 2500HS is a widefield high-content imaging system designed for speed and throughput. Equipped with deconvolution, IN Cell Analyzer 2500HS is optimized for image quality and long-term live-cell imaging.

    • Biological relevance: Optimized hardware and features for 3D and live-cell imaging
    • Deconvolution: Increased contrast with less excitation power improves image quantitation and avoids artifacts associated with photodamage and phototoxicity in live cells.
    • Smart scanning: Spend less time imaging and reduce data load by only imaging the objects of interest.
    • Speed and sensitivity: Higher throughput and faster frame rates for single and multichannel imaging.

    A complete widefield HCA solution designed for throughput and image quality

    Designed for high-throughput, speed, and sensitivity, IN Cell Analyzer 2500HS is a fourth-generation fully automated widefield cell imaging system from Cytiva. Building on the capabilities of earlier IN Cell Analyzer systems, it provides an easily accessible platform for high-content analysis (HCA), high-throughput screening, and large-scale automated live-cell imaging. IN Cell Analyzer 2500HS has all the features of a widefield imaging system with the benefits of automation for ease of use. Multiple hardware and software improvements increase speed and sensitivity to address the challenges of generating meaningful results from dim and live samples. Combined with new IN Carta software, which simplifies image analysis workflows, IN Cell Analyzer 2500HS enables users at all levels to perform powerful analysis as part of a complete HCA solution from assay to answer.

  • ParameterIN Cell Analyzer 2500HS
    AutomationCompatible with most commercially available laboratory automation systems 
    Height66cm / 26in 
    Width112.8cm / 44.4in mm
    Depth63.5cm / 25.0in 
    Weight105.7kg / 233lbs kg
    ObjectivesRoom for up to four objectives
    Supplied with 10×/0.45 Objectives available from 2-100x (optional)
    Both air and water objectives available 
    Light SourceSeven-color Solid State Illuminator (SSI) for fluorescence excitation
    White LED for transmitted light 
    Imaging ModesWidefield (open aperture)
    2D deconvolution, Advanced 2D deconvolution, 2.5D, 3D deconvolution (optional)Transmitted light
    DIC (optional)
    Phase (optional) 
    Pack size

  • Water Immersion objectives now included

    As the transition to more complex imaging applications speeds up, so does the need for improved light efficiency, resolution and flexibility of instrumentation. Imaging thick 3D cultures imbedded in aqueous media requires non-air objectives with high numerical aperture and minimal spherical aberration. Water immersion objectives are ideal to achieve maximal image quality for such samples, while reducing the scan times due to better resolving power.

    Biological relevance

    IN Cell Analyzer 2500HS is designed to eliminate systematic variability and give you confidence in your results. With reliable autofocus under all conditions, vibration isolation, and environmental control designed for uniformity, you can be assured that your data is a true representation of the biological phenomena in question.


    High contrast is critical when automated image analysis is generating your data. The higher the contrast, the more reliable the segmentation and quantitation. Deconvolution on IN Cell Analyzer 2500HS reassigns background signal to the biological structure of interest to boost image contrast while preserving the quantitative information you need for reliable analysis.

    Smart scanning

    The difference between a standard microscope and an automated HCA system built for screening is primarily a person's intervention and their time. A common approach to solving this problem is to image everything. Smart scanning features on the IN Cell Analyzer 2500HS can automate the human element and reduce the total time and data load by finding structures of interest and inspecting them in more detail.

    Speed and sensitivity

    Technology is constantly evolving and the IN Cell Analyzer platforms are keeping pace. With the latest sCMOS camera technology the IN Cell Analyzer 2500HS provides lower noise, shorter exposure times, and faster frame rates. In addition, hardware optimizations tailored to multichannel experiments increase both speed and throughput for 2D and 3D experiments.