Designed for chemiluminescence detection and gel documentation
This CCD imager is a practical and affordable solution to individual
researchers or laboratories that are either looking for their own imager
or need extra imaging capacity. The 30 × 28 cm footprint has the same
area as a typical laptop computer, which allows the imager to fit neatly
on almost any lab bench without the need to connect ancillary
equipment.
Ready for use in less than five minutes
ImageQuant LAS 500 imager consists of a touchscreen, sample chamber,
USB port, camera and light sources. The 8.3-megapixel CCD camera is
ready for use in less than five minutes. Reliable Peltier cooling
combined with binning significantly reduces noise levels and improves
both the sensitivity and linearity of the system.
Flexible imaging modes for Optimal chemiluminescence detection
Signal intensity can be detected either automatically or
semi-automatically by specifying an area of interest. In addition,
single (manual) or multiple (incremental) exposures can be set by the
operator. The camera is designed for optimal chemiluminescence detection
with regard to sensitivity and dynamic range.
Three LED light sources
The system features three LED light sources: a combined blue epi-/UV
epi-light and a white epi-light. The combined blue epi-/UV epi-light
allows imaging of fluorescence signals above 550 nm. This feature makes
the camera well suited for DNA stain imaging with SYBR™ Green, ethidium
bromide, or Deep Purple Total Protein Stain.
A fast and easy way to verify the molecular weight of detected protein bands
The white epi-light source (470 to 635 nm) enables documentation of
colorimetric markers and stains, such as Coomassie Blue or Silver.
Moreover, the white light mode can be combined with the
chemiluminescence mode to automatically generate an overlay image of the
colored marker and chemiluminescent sample images. This provides a fast
and easy way to verify the molecular weight of detected bands, and
identification of band of interest if multiple bands are generated.
Software
In addition to the on-board software, images acquired using
ImageQuant LAS 500 can be analyzed using ImageQuant TL (IQTL). IQTL is
an automated and easy-to-use general-purpose gel, blot, and array image
analysis software. IQTL requires installation on an external computer
and cannot be installed on ImageQuant LAS 500.
Western blot detection
Western blotting, also known as immunoblotting, or protein blotting,
is a well-established and widely used technique for the detection and
analysis of proteins. A variety of detection systems, based on
chemiluminescence, chemifluorescence, fluorescence, chromogenic, or
radioisotopic detection are available. Chemiluminescence is reliable and
well known for having the greatest sensitivity of any available
detection method.
Further, there has been a decline in usage of radioisotopic and
chromogenic reagents due to safety issues with handling radioactive
isotopes and poor sensitivity with chromogenic reagents, which has
meant, enzyme-based chemiluminescent and direct fluorescence have been
extensively developed and are now the preferred method for detection due
to their improved sensitivity and wider dynamic range.
What is chemiluminescence western blot detection?
Chemiluminescence Western Blot detection is based on antibodies
conjugated to horseradish peroxidase (HRP) that catalyze the oxidation
of luminol in presence of peroxide, resulting in light emission. HRP has
several advantages over other enzymes such as alkaline phosphatase
(AP). HRP can be easily conjugated to antibodies or streptavidin (which
binds with extraordinarily high affinity to biotin, a commonly used tag,
and can be used with different chemiluminescent reagents. Considerable
efforts have been made to develop HRP-based detection reagents to obtain
higher detection sensitivity, stronger light intensity and long-lasting
signals.
Equipment services
OptiRun service solutions
offer a comprehensive range of options that can be customized to your
asset-management needs and desired outcomes. Learn more about service
agreements and lifecycle support for your equipment.