The new SPH1299 UHPLC pump is a robust,
precise and easy to use gradient pump. It delivers highly precise flow
rates at up to 1300 bar. With its fully automatic compensation of
compressibilities and easy handling, the SPH1299 is state of the art. A
new concept for solvent suction makes pump preparation child's play.
pump consists of two pairs of serial pump heads. Each of the four pump
heads can be controlled individually. This offers the highest
flexibility for flow control and the best conditions for optimal pulse
Once set to a defined flow rate, the SPH1299 maintains it exactly -
completely independent of solvent composition and pressure. The
compressibility of the solvent is measured, based on the measurements,
deviations due to flow pulsation and flow rate reduction due to
compression are compensated. By automatically determining the
compressibility of the solvent, the pulsation of the flow is reduced.
Especially UHPLC methods can be optimally processed and transferred by
this feature of the SPH1299.
The SPH1299 has an active piston backwash.
This protects the piston seals and increases the running time of the
entire pump. For the occasional replacement of the piston seals, which
is nevertheless necessary, the system has a very simple mechanism.
Within a few minutes the pump head is simply unplugged, removed and the
seal replaced. The pump and possible covers can remain in place.
SPH1299 UHPLC pump is very well suited for Axel Semrau's multiplexing
solution for better LC-MS utilization. Here the target analytes from
several HPLC lines are fractionated on one LC-MS, so that the throughput
can be increased and the matrix load reduced.