A flexible and fully-automated oligonucleotide synthesizer
ÄKTA oligopilot plus is a flexible and fully-automated DNA/RNA
oligonucleotide synthesizer designed for use in research and for process
development. ÄKTA oligopilot plus is designed to meet the needs of
various oligonucleotide chemistries and therapeutics (RNAi, siRNA,
aptamer, antisense, immunostimulating CpG motifs, Exon skipping)
enabling cost-efficient synthesis of high-quality oligonucleotides.
Flow-through reactor technology
The system employs flow-through reactor technology, which results in
significantly reduced reagent consumption, enables exact control of
reaction rates and contact times, and allows smooth scale-up.
Column re-circulation
ÄKTA oligopilot plus features column re-circulation, an important
feature for extending the time of coupling reactions, of significant
impact when coupling RNA or different specific modified amidites. ÄKTA
oligopilot plus is available in two different configurations: ÄKTA
oligopilot plus 10 for synthesis in the 10 - 50 μmol range and ÄKTA
oligopilot plus 100 for synthesis in the 50 μmol - 9 mmol range.
Fastest possible column wash
Pressure controlled flow and conditional method programming based on
UV signals allows the fastest possible column wash between synthesis
steps and reduces synthesis cycle time. The batch operation and
documentation are in accordance with the requirements of current good
manufacturing practice (cGMP), good laboratory practice (GLP), and is in
full compliance with 21 CFR Part 11.
Compatible with a range of column reactors
ÄKTA oligopilot plus is compatible with a range of column reactors
ranging from fixed column volume 1.2 mL – 48 mL to larger, adjustable,
column volumes of FineLINE 35 oligo (scales from 250 μmol - 4 mmol) and
the Adjustable column (<9 mmol). Monomer sliders and bottle caps have
been developed for use with ÄKTA oligopilot plus. The sliders fit on
existing mounting rails on the instrument side. Bottle caps have one gas
connection, and three reagent connections that fit all different
capillaries used with the system.
About UNICORN control software
ÄKTA oligopilot plus is controlled via an external computer using
UNICORN software especially designed for production needs. Features such
as a sequence editor for up to 200 bases, password protection for
different access levels, lot number registration, and full synthesis
documentation are available. Combining ÄKTA oligopilot plus extensive
monitoring capabilities with UNICORN allows a PAT (Process Analytical
Technology) approach. Online trityl monitoring and coupling efficiency
display provide security during synthesis, shutting down the system if
coupling efficiency drops below a pre-determined threshold.